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Immortalized Human Ovarian Epithelial Cells-SV40

Cat.No.: CSC-I9009L

Species: homo sapiens

Source: Ovaries

Morphology: Polygonal (at low density), Cuboidal (at high density)

Culture Properties: Adherent

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Cat.No.
CSC-I9009L
Description
Immortalized Human Ovarian Epithelial Cells-SV40 have been obtained immortalizing Human Primary Ovarian Epithelial Cells with LentiSV40 Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40tranduced cells go beyond 30 passages.
Species
homo sapiens
Source
Ovaries
Recommended Medium
SuperCult® Immortalized Human Ovarian Epithelial Cell Medium (Cat No.: CM-I9009L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal (at low density), Cuboidal (at high density)
Immortalization Method
SV40 large T antigen
Application
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C4876L Human Ovarian Epithelial Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

The origin of epithelial ovarian cancer has long been a matter of debate, with proposed sources including ovarian surface epithelial (OSE) cells, secondary Müllerian tract structures, or fallopian tube epithelium. Despite being the second most common type of gynecological cancer and the leading cause of death in the United States, in vitro cell models that mimic normal ovarian epithelial cells and their malignant counterparts are lacking.

Primary ovarian cells can only be maintained in vitro for a few passages before becoming senescence or undergoing spontaneous transformation. Cells immortalized with tumor antigens can be grown as cell lines, but an obvious concern is that their properties may be altered by tumor antigen-induced cell transformation. A deeper understanding of the role of ovarian stroma in ovarian biology and carcinogenesis requires the availability of ovarian cell lines that can continuously grow in vitro but are not transformed. To address this gap, we established immortalized human OSE (IHOSE) cell lines that have stable growth without malignant properties. The epithelial characteristics were confirmed by cytokeratin 7 and cytokeratin 18 marker expression. Confirmation of these characteristics and stability of IHOSE cell line could provide a useful resource for comparative or gene expression studies on ovarian cancer, helping to identify novel therapeutic and/or diagnostic targets.

Identification and Characterization of Multipotential Stem Cells in Immortalized Normal Ovarian Surface Epithelial Cells

The IOSE80 cell line represents immortalized cells of the ovarian epithelium. These cells were obtained from normal human ovarian epithelial cells that had been transfected with SV-40T. It not only maintains some morphological characteristics and molecular markers of normal ovarian epithelium but also has strong proliferation ability in vitro. It is commonly used to study the origin of epithelial ovarian cancer or as a control for epithelial ovarian cancer cell research. However, its characteristics have not yet been fully identified. This study aims to determine whether IOSE80 have the characteristics of stem cell proliferation and multilineage differentiation and their application in regenerative medicine.

The IOSE80 cell line has the characteristics of ovarian epithelial cells.Fig. 1. Characteristics of the IOSE80 cell line (Hou, Lin, et al. 2024).

The IOSE80 cell line has the characteristics of PSCs (IPS cells and ES cells, respectively)Fig. 2. Comparison of transcriptomic differences between IOSE80 cells and pluripotent stem cells (Hou, Lin, et al. 2024).

SMS2 But Not SMS1 is Upregulated in Ovarian Cancer Cells and SMS2 Overexpression Promotes Growth and Migration in Immortalized Normal Ovary Epithelial Cells

Sphingomyelin synthase 1 (SMS1) and 2 (SMS2) are two enzymes required for sphingomyelin de novo synthesis, and their roles in tumor transformation and development have been recently recognized. In this work, we systematically evaluated the expression patterns of SMS1 and 2 in ovarian cancer patient samples and cell lines. Furthermore, we analyzed the functions of SMS2 and its underlying mechanisms.

We found that 6 of 7 ovarian cancer cell lines demonstrated higher SMS2 protein levels than IHOEC (human normal ovary epithelial cell immortalized with SV40 lentiviral transfection) (Fig. 3A). The upregulation of SMS2 but not SMS1 in ovarian cancer cells was not only on the protein level but also seen on the mRNA level (Fig. 3B). We next transfected IHOEC with mammalian expression construct containing SMS2 and confirmed SMS2 overexpression (Fig. 3C). We further found that SMS2 overexpression significantly increased proliferation and migration in IHOEC (Fig. 3D, 3E). Of note, proliferation and migration were increased by ~1.5-fold and ~5-fold respectively, suggesting that SMS2 might play a predominant role in migration rather than proliferation.

Analysis of SMS1 and SMS2 in ovarian cancer cells compared with normal immortalized human ovarian epithelial cells (IHOEC). Analysis of SMS2 level in IHOEC cells after transfection of SMS2-overexpressing plasmid.Fig. 3. SMS2 overexpression promotes growth and migration in immortalized human ovarian epithelial cells (IHOEC).

What is immortalization?

Primary cells reach a state of replicative senescence after a certain amount of proliferation, and a certain number of divisions, thus limiting the proliferation of senescent or damaged cells. GeneCopoeia provides cell immortalization reagents that induce immortalization using recombinant lentiviral-carrying genes, such as simian virus 40 (SV40) large T-antigen and human telomerase reverse transcriptase protein (hTERT), can significantly extend the lifespan of primary cells.

What are Immortalized Human Ovarian Epithelial Cells-SV40?

Immortalized Human Ovarian Epithelial Cells-SV40 are ovarian epithelial cells that have been transformed using the SV40 large T antigen, allowing them to proliferate while retaining key characteristics of normal ovarian epithelial cells. These models are essential for studying ovarian biology and related diseases.

What research applications are Immortalized Human Ovarian Epithelial Cells-SV40 suitable for?

These cells are widely applicable and can be used in various research fields, including:
Ovarian cancer research and drug response studies.
Investigating ovarian physiology and hormone regulation.
Studies on cell signaling pathways in ovarian cells.
Research on epithelial-to-mesenchymal transition (EMT) in cancer.
Toxicology assessments regarding ovarian toxicity.

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