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Immortalized Human Microglia-SV40
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Note: Never can cells be kept at -20 °C.
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
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If most of the cells are attached back to the bottom of the bottle, it shows that the cell viability is normal, the remaining floating cells can be removed, leaving 8-10 mL culture medium culture observation, cell growth to confluence 85-95%, digestion and passaging, such as the cells are still not attached to the wall, the cells will be centrifuged and collected to a new culture bottle, the original bottle plus part of the medium to continue to cultivate, in the middle of the attention to observation, our technicians will be followed up and guidance until the problem is solved, and then the cells can be transferred back to the bottom of the bottle. Our technicians will follow up and guide until the problem is solved.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Reduced contamination
I change the culture medium promptly during the cultivation of the cells, which greatly reduces the probability of contamination.
13 Apr 2022
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