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Immortalized Human Gingival Keratinocytes (Gie-No3B11)
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Cat.No.
CSC-I9236L
Description
The gingival epithelium protects periodontal tissue from bacteria and toxic substances. Keratinocytes play an important role in promoting strong epithelial bonds through the formation of intercellular connections with the cytoskeleton and neighboring cells. The Immortalized Human Gingival Keratinocytes are isolated primary oral keratinocytes transfected to express the HPV-16 E7. It expresses cytokeratin 2, cytokeratin 17, cytokeratin 19, involucrin, filaggrin, and claudin-1. These cells may be differentiated by altering culturing conditions to build transepithelial electrical resistance, a distinct characteristic of gingival keratinocytes. These cells are recommended for studies in mechanisms that contribute to the specific properties of the gingiva tissue, such as the epithelial barrier function.
Species
Homo sapiens
Source
Buccal Gingiva
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and chemical mediated transfection with plasmid carrying human papilloma virus (HPV-16) E6 and E7 genes
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
Flow cytometry, western blot, and immunofluorescence detected cell marker expression.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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