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Immortalized Human Endoneurial Endothelial Cells (THEndEC)
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Cat.No.
CSC-I9212L
Description
As part of the blood-nerve barrier (BNB), the endoneurial endothelial plays a significant role in the regulation of influx and efflux of water, nutrients and xenobiotics that is needed to maintain the peripheral nerve internal microenviroment.The Immortalized Human Endoneurial Endothelial Cells (THEndEC) is a useful model for use in facilitated receptor-mediatedin vitroBNB permeability and/or kinetic studies, in addition to susceptibility to drug intoxication research as it retained specialized transporters (i.e. P-gp, γ-GT, LAT-1, CRT and MCT-1) and restrictive barrier function similar to primary endoneurial endothelial cells. The THEndEC has been characterized to show specific binding to UEA-1 lectin and the ability of Dil-Ac-LDL uptake, indicative of α-L-fucose expression and specialized scavenger receptor expression, respectively. Further studies demonstrated transendothelial electrical resistance (TEER) and permeability to fluoresceinated sodium and dextran using THEndEC, making this cell line suitable for peripheral nerve permeability studies as well.
Species
Homo sapiens
Source
Sciatic nerve
Culture Properties
Adherent
Morphology
spindle shaped
Immortalization Method
Serial passaging and stable transfection with a plamid carrying SV40 Large T antigen
Markers
GLUT-1, P-gp, γ-GT, LAT-1, CRT and MCT-1
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
1) Immunocytochemistry was used to confirm the nuclear expression of SV40-LTA in the immortalized cells
2) RT-PCR and western blot were used to assess the expression of CRT, GLUT-1, P-gp, LAT-1, MCT-1 and γ-GT;
3) Transendothelial electrical resistance (TEER) assessment was performed to evaluate the biophysical property of the immortalized cells;
4) Sodium fluorescein and FITC-dextran were used to evaluate the solute permeability of the immortalized cells;
5) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI) -labelled Ac-LDL was used to evaluate Ac-LDL uptake.
2) RT-PCR and western blot were used to assess the expression of CRT, GLUT-1, P-gp, LAT-1, MCT-1 and γ-GT;
3) Transendothelial electrical resistance (TEER) assessment was performed to evaluate the biophysical property of the immortalized cells;
4) Sodium fluorescein and FITC-dextran were used to evaluate the solute permeability of the immortalized cells;
5) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI) -labelled Ac-LDL was used to evaluate Ac-LDL uptake.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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