Featured Products
Our Promise to You
Guaranteed product quality, expert customer support
Immortalized Human Endometrial Stromal Cells (HESC)
- Specification
- Recommended Products
Cat.No.
CSC-I9213L
Description
Decidualization of the endometrium, mainly induced by progesterone, is a process of endometrial remodelling where the endometrial stromal cells differentiate into secretory decidual cells in preparation for pregnancy. Decidualized endometrial stromal cells are characterized by release of prolactin (PRL), insulin-like growth factor binding protein-1 (IGFBP-1), tissue factor (TF), plasminogen activator inhibitor-1 (PAI-1), and expression of basal lamina components such as laminin, collagen IV, and fibronectin.The Immortalized Human Endometrial Stromal Cells (HESC) is unique in that 1) it displays normal karyotype and 2) it responds to hormone stimulation and retains the morphological pattern and biochemical endpoints of decidualization after treatment of estradiol and medroxyprogesterone, making this cell line a valuable tool in endometrium homeostasis and female reproductive studies.
Species
Homo sapiens
Source
Endometria
Culture Properties
Adherent
Morphology
Fibroblast-like
Immortalization Method
Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line
Markers
IGFBP-1, PRL, TF, PAI-1 at decidualization endpoints
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Quality Control
1) TRAPezeELISA was used to detect the telomerase activity in the immortalized cells
2) ELISA and RT-PCR were performed to detect IGFBP-1, FN, PRL and PAI-1 secretion and mRNA expression;
3) Western blot and densitometry were conducted to determine the expression of TF and Fas/Fas ligand.
2) ELISA and RT-PCR were performed to detect IGFBP-1, FN, PRL and PAI-1 secretion and mRNA expression;
3) Western blot and densitometry were conducted to determine the expression of TF and Fas/Fas ligand.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Customer Support & Price Inquiry
