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Immortalized Human Corneal Fibroblasts (IHCF)
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Cat.No.
CSC-I9206L
Description
Tissue repair and wound healing in the cornea is accomplished mainly by the corneal fibroblasts, and these cells are responsible for pathological process in the degenerative corneal disorders such as keratoconus. Established using the HPV16 E6/E7 genes, The Immortalized Human Corneal Fibroblasts (IHCF) not only retains its authentic cell biomarkers (CD36, ECM protein collagen I and S100A4), it also displays fairly disomic cytogenetic status at chromosomes 1, 8, 10 and 18, which is an indication of normal diploid chromosomal status.Together with the Immortalized Human Corneal Keratinocytes and the Immortalized Human Corneal Endothelial cells isolated from the same donor and immortalized using the same platform, this uniform model system presents a valuable tool for studies involving cornea homeostasis and ophthalmologic diseases.
Species
Homo sapiens
Source
corneoscleral rim
Culture Properties
Adherent
Morphology
spindle shaped
Immortalization Method
Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene
Markers
CD36, ECM protein collagen I, S100A4
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
1) RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7;
2) Indirect immunofluorescence was performed to detect the cell specific marker expression CD36, ECM protein collagen I, and S100A4; 3) FISH was used to examine the cytogenetic alterations in the immortalized cells.
2) Indirect immunofluorescence was performed to detect the cell specific marker expression CD36, ECM protein collagen I, and S100A4; 3) FISH was used to examine the cytogenetic alterations in the immortalized cells.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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