SIMA

Cat.No.: CSC-C3438

Species: Homo sapiens (Human)

Source: Adrenal Gland

Morphology: cell shape is bipolar, cells growing as monolayer or forming foci

Culture Properties: monolayer

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Cat.No.

CSC-C3438

Description

Established from the adrenal tumor tissue resected after treatment from a 20-month-old boy of European origin with neuroblastoma (stage III) in 1991

Species

Homo sapiens (Human)

Source

Adrenal Gland

Recommended Medium

90% RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

cell shape is bipolar, cells growing as monolayer or forming foci

Karyotype

Human hypotetraploid karyotype with 3.5% polypoidy - 88/89<4n>XXY/XXYY, -Y, -11, -17, -17, der(1qh++)t(1;17)(p34-35;q12)x2, t(6;9)(p21;q33)x2, ?inv(12)(q21q24)x2, der(22)t(17;22)(q21;p12)x2 - ca. 50-100 NMYC-containing dmin present in each cell - t(1;17)

Disease

Adrenal Gland Neuroblastoma

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: ELISA: reve

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

SiMa

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.