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SIMA
- Specification
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Cat.No.
CSC-C3438
Description
Established from the adrenal tumor tissue resected after treatment from a 20-month-old boy of European origin with neuroblastoma (stage III) in 1991
Species
Human
Source
neuroblastoma
Recommended Medium
Culture Properties
monolayer
Morphology
cell shape is bipolar, cells growing as monolayer or forming foci
Karyotype
Human hypotetraploid karyotype with 3.5% polypoidy - 88/89<4n>XXY/XXYY, -Y, -11, -17, -17, der(1qh++)t(1;17)(p34-35;q12)x2, t(6;9)(p21;q33)x2, ?inv(12)(q21q24)x2, der(22)t(17;22)(q21;p12)x2 - ca. 50-100 NMYC-containing dmin present in each cell - t(1;17)
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: ELISA: reve
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: ELISA: reve
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Why should the medium be washed with PBS in cell culture?
A:
The pH value of PBS buffer is between 7.2 and 7.4, which is the most suitable pH range for cells. And the pH value will remain largely unchanged under stimulation by trace amounts of acid and base.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Good quality control
The quality control of the cell products is very good, as demonstrated by the good reproducibility and the ease of handling steps.
13 Feb 2021
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