Description
Established by Seeger et al., (1977) from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma. The cells are neuroblastic, tear-drop shaped with multiple short fine cell processes (neurites). The cells are tumourigenic in nude mice. This is a catecholamine producing neuroblastoma cell line.LA-N-1, LA1-55n and LA1-5s have been shown to originate from the same patient by STR profiling.
Tissue of Origin
Neuroblastoma
Recommended Medium
EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Storage
Liquid Nitrogen (-180 °C).
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What happens to the culture medium after mycobacterial contamination during cell culture?
Generally, the culture medium does not become cloudy; however, it is easier to notice dotted colonies (yellowish or white) floating on the surface of the medium with the naked eye.