LNCaP clone FGC; LNCaP.FGC

Cat.No.: CSC-C9478L

Species: Homo sapiens (Human)

Source: Lymph Node Metastasis

Morphology: epithelial

Culture Properties: adherent, single cells and loosely attached clusters

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Cat.No.

CSC-C9478L

Description

These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase); the cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared; they attach only lightly to the substrate, do no become confluent and rapidly acidify the medium; growth is very slow; the cells should be allowed to incubate undisturbed for the first 48 hours after subculture; when flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium. Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach. The medium can then be removed and replaced with fresh medium. If desired the contents of the flask can be collected, centrifuged at 300xg for 15 minutes, resuspended in 10ml of medium and dispensed into a single flask.

Species

Homo sapiens (Human)

Source

Lymph Node Metastasis

Recommended Medium

90% RPMI-1640 and 10% h.i. FBS

Culture Properties

adherent, single cells and loosely attached clusters

Morphology

epithelial

STR DNA Profile

D3S1358: 16
vWA: 16,18
FGA: 19,20
Amelogenin: X,Y
TH01: 9
TPOX: 8,9
CSF1P0: 10,11
D5S818: 11,12
D13S317: 10,12
D7S820: 9,11

Karyotype

pseudodiploid male; seven marker chromosomes

Disease

Prostate Carcinoma

Storage and Shipping

liquid nitrogen vapor phase

Synonyms

LNCaP clone FGC; LNCaP-Clone-FGC; LNCaP.FGC; LNCaP-FGC; LNCaP FGC; LNCAPCLONEFGC; LNCaP-ATCC; LNCaP Fast Growing Colony

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.