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LNCaP clone FGC; LNCaP.FGC
- Specification
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Cat.No.
CSC-C9478L
Description
These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase); the cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared; they attach only lightly to the substrate, do no become confluent and rapidly acidify the medium; growth is very slow; the cells should be allowed to incubate undisturbed for the first 48 hours after subculture; when flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium. Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach. The medium can then be removed and replaced with fresh medium. If desired the contents of the flask can be collected, centrifuged at 300xg for 15 minutes, resuspended in 10ml of medium and dispensed into a single flask.
Species
Homo sapiens (human)
Tissue of Origin
Prostate
Culture Properties
adherent, single cells and loosely attached clusters
Morphology
epithelial
STR DNA Profile
D3S1358: 16
vWA: 16,18
FGA: 19,20
Amelogenin: X,Y
TH01: 9
TPOX: 8,9
CSF1P0: 10,11
D5S818: 11,12
D13S317: 10,12
D7S820: 9,11
vWA: 16,18
FGA: 19,20
Amelogenin: X,Y
TH01: 9
TPOX: 8,9
CSF1P0: 10,11
D5S818: 11,12
D13S317: 10,12
D7S820: 9,11
Karyotype
pseudodiploid male; seven marker chromosomes
Storage and Shipping
liquid nitrogen vapor phase
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What types of specimens are suitable for TEM imaging?
A:
TEM can be used to image a wide range of specimens, including cells and tissues. However, samples need to be prepared as thin sections (<100 nm) to allow the transmission of electrons through them.
Average Rating: 4.0 | 1 Scientist has reviewed this product
Advancing the research process
These tumor cell products have greatly advanced our cancer treatment research.
10 Mar 2023
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