Cytogenetic analysis and DNA fingerprinting at the Creative Bioarray documented that this cell line is identical with the human prostate carcinoma-derived cell line DU-145, but shows some phenotypical differences; DU-145 was established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975
Human hypotriploid karyotype with 22% polyploidy - 59(55-63)<3n>X, -X/Y, -Y, -2, -3, -4, -7, -8, -14, -16, -19, -20, -21, -22, +5mar (inc bisat x2-3), del(1)(p31), del(2)(p12), i(5p), del(6)(q22)/add(6)(q?12), der(6)t(4;6)(q22;p22.3), del(9)(p21), add(10)
epithelial-like cells growing as monolayers
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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