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Immortalized Mouse Microglia (SIM-A9)
Cat.No.: CSC-I9208L
Species: Mus musculus
Source: Postnatal mouse cerebral cortices
Culture Properties: Adherent
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Note: Never can cells be kept at -20 °C.
2) ELISA was used to determine the TNFα secretion after inflammatory stimulation;
3) Western blot and immunocytochemistry were used to confirm the expression of microglial specific markers such as CD68 and Iba1 and M1/M2 phenotype-associated protein expression (COX-2/iNOS and Arg-1) after stimulation;
4) Aβ1-42orE. coli-derived bioparticles uptake was used to determine phagocytic activity of SIM-A9 cells.
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