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Immortalized Mouse Cerebellar Capillary Endothelial Cells (cerebEND)
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Cat.No.
CSC-I9185L
Description
The mammalian cerebellum is indispensable in the coordination of voluntary movement (i.e. balance and locomotion), muscular tonus and equilibrium. The cerebellar blood brain barrier (BBB) helps maintain the homeostasis of the cerebellum microenvironment and its integrity is often connected to many disorders of the central nervous system (CNS). The Immortalized Mouse Cerebellar Capillary Endothelial Cell Line (cerebEND) provides a useful model for studies involving the differentiation and regulation of BBB as it shares principal features of the BBBin vivo, specifically in that 1) it retains endothelial markers VE-Cadherin and PECAM-1; 2) it displays tight junction associated markers such as occludin, claudin-3, -5, -12, 3) it expresses the BBB marker protein Glut-1 and 4) it shows high electrical resistance, representing barrier properties. This cell line is also responsive to glucocorticoid, estrogen-treatment and pro-inflammatory mediator such as TNFα, making this cell line valuable in elucidating cellular responses of endothelial cells to different stimuli. Compared to the Immortalized Mouse Cerebral Capillary Endothelial Cell Line (cEND), the cerebEND shows a higher fragility of the cerebellar BBB to inflammatory stimuli; together the two cell lines represent important model systems for these different brain regions.
Species
Mus musculus
Source
Cerebellar cortex
Culture Properties
Adherent
Morphology
spindle shaped
Immortalization Method
Transformation with oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT)
Markers
Claudin-5, Occuldin, Glut-1, VE-Cadherin
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Endothelial marker VE-Cadherin and PECAM-1 assessed by immunostainning;
2) Tight junction protein Claudin-5 assessed by immunostainning and claudin-1, -3 and -12 detected in mRNA levels;
3) BBB marker protein Glut-1 and tight junction-associated protein occludin measured by western blot analysis.
The cell line's response to pro-inflammatory stimuli was measured by 1) TER, 2) western blot and 3) gene expression analysis using RT-PCR.
2) Tight junction protein Claudin-5 assessed by immunostainning and claudin-1, -3 and -12 detected in mRNA levels;
3) BBB marker protein Glut-1 and tight junction-associated protein occludin measured by western blot analysis.
The cell line's response to pro-inflammatory stimuli was measured by 1) TER, 2) western blot and 3) gene expression analysis using RT-PCR.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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