Description
Established in 1990 from the right axillary lymph node of a 48-year-old woman with metastatic malignant melanoma in the right chest
Karyotype
Human hypotriploid karyotype with 20% polyloidy - 65(61-69)<3n>XXX, -4, -5, -9, -10, -12, +2mar, add(4)(p11), del(6)(p21), del(7)(p14)x2
Morphology
adherent fibroblasts, not limited to monolayer growth
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
