COLO-783

Cat.No.: CSC-C0357

Species: Human

Source: melanoma

Morphology: adherent fibroblasts, not limited to monolayer growth

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Cat.No.

CSC-C0357

Description

Established in 1990 from the right axillary lymph node of a 48-year-old woman with metastatic malignant melanoma in the right chest

Species

Human

Source

melanoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Morphology

adherent fibroblasts, not limited to monolayer growth

Karyotype

Human hypotriploid karyotype with 20% polyloidy - 65(61-69)<3n>XXX, -4, -5, -9, -10, -12, +2mar, add(4)(p11), del(6)(p21), del(7)(p14)x2

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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