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LCL-HO
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Cat.No.
CSC-C3441
Description
This lymphoblastoid cell line was established by EBV transformation of peripheral blood lymphocytes; cell line was submitted as autologous to cell line MEL-HO that was derived from a woman with primary melanoma in 1976; however, cytogenetic and DNA fingerprinting analysis showed clearly that LCL-HO and MEL-HO are not related
Species
Human
Source
B lymphoblastoid cells
Culture Properties
suspension
Morphology
lymphoblastoid-like cells growing in suspension singly or in clumps
Karyotype
Human diploid karyotype with 2% polyploidy and near triploid sideline (4%) - 46(45-47)<2n>XX, der(12)t(12;?6)(q24.32;p21.2) - additional pseudodiploid subclones are present with other structural and numerical rearrangements
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cy IgM -, cykappa -, cylambda +
Viruses: ELISA: reverse
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cy IgM -, cykappa -, cylambda +
Viruses: ELISA: reverse
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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