Established from the primary colorectal cancer (TNM stage 3) of the right colon of a woman; cells were described to be well differentiated
adherent epithelial-like cells growing as monolayers and in aggregates
Human hyperdiploid karyotype with 8% polyploidy - 48(45-49)<2n>XX, +12, +13 - no consistent structural abnormality was detected
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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