Established from a woman with a moderately differentiated primary adenocarcinoma of the right colon (TNM stage 2)
Human flat-moded hypodiploid karyotype with 16%polyploidy - 45(42-52)<2n>X/XX, -6 - sdl with +3, del(3)(q21) - near-normal "unstable" karyotype with many inconsistent structural and numerical changes
epithelial-like cells growing as monolayer; cells do not grow to complete confluency
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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