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Cat.No.
CSC-C0449
Description
Established from the tumor of a 52-year-old Japanese man with highly differentiated squamous cell carcinoma of the lower alveolus which was highly invasive to the mandibular bone and the muscle layer of the oral floor; described as anchorage-independent and to be tumorigenic in nude mice
Species
Human
Source
oral squamous cell carcinoma
Recommended Medium
90% DMEM + 10% h.i.FBS
Culture Properties
monolayer
Morphology
epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogenous) growing in monolayers
Karyotype
Human flat-moded hypotriploid karyotype with 28% polyploidy - 61-68<3n>XX, -Y, -1, -2, +4, -5, -6, +9, +11, -13, +15, -17, -18, +20, +20, -21, +3-4mar, del(X)(q11q21), add(1)(q11), ?inv(2)(p17q11), add(2)(q11), del(3)(p11p14.3)x2, i(4p), add(4)(q3?1), del
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokerati
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CM-1010X

For research use only. Not for any other purpose.

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Q:
Why is L-glutamine used in media?
A:

L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing.

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Average Rating: 4.0    |    1 Scientist has reviewed this product

Highly recommended

By culturing these cells with other supportive cell types, we can recreate and study the complex interactions occurring within a tumor niche. Highly recommended!

04 May 2023


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