BHY

Cat.No.: CSC-C0449

Species: Human

Source: oral squamous cell carcinoma

Morphology: epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogenous) growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0449

Description

Established from the tumor of a 52-year-old Japanese man with highly differentiated squamous cell carcinoma of the lower alveolus which was highly invasive to the mandibular bone and the muscle layer of the oral floor; described as anchorage-independent and to be tumorigenic in nude mice

Species

Human

Source

oral squamous cell carcinoma

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogenous) growing in monolayers

Karyotype

Human flat-moded hypotriploid karyotype with 28% polyploidy - 61-68<3n>XX, -Y, -1, -2, +4, -5, -6, +9, +11, -13, +15, -17, -18, +20, +20, -21, +3-4mar, del(X)(q11q21), add(1)(q11), ?inv(2)(p17q11), add(2)(q11), del(3)(p11p14.3)x2, i(4p), add(4)(q3?1), del

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokerati

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why is L-glutamine used in media?

L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Highly recommended

By culturing these cells with other supportive cell types, we can recreate and study the complex interactions occurring within a tumor niche. Highly recommended!

04 May 2023

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