Description
Established from the tumor of a 60-year-old Japanese man with moderately differentiated squamous cell carcinoma of the soft palate which was invasive into muscle layer; cell line was established from a cervical lymph node metastasis which occurred seven years after initial treatment of the primary tumor; the tumor metastasized not only to the lungs, but also to the brain; described as anchorage-independent and tumorigenic in nude mice
Organism
Homo sapiens ,human
Source
oral squamous cell carcinoma
Morphology
epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogeneous) growing in multilayers
STR DNA Profile
Amelogenin: X,X
CSF1PO: 11
D13S317: 10
D16S539: 12,13
D18S51: 13
D21S11: 29,30
D3S1358: 15
D5S818: 12
D7S820: 10
D8S1179: 13
FGA: 23
Penta D: 10,13
Penta E: 16
TH01: 9
TPOX: 9,11
Vwa: 17
Karyotype
Human flat-moded hypotriploid karyotype with 4% polyploidy - 60-64<3n>XX, -Y, +1, -2, -4, +5, -6, +7, -10, -12, -13, -13, -17, -18, -18, -21, -22, +3-5mar, add(X)(p22), der(1)add(1)(p36)ins(1;?)(q11;?), t(4;14)(q21;p11), i(4p)x1-2, del(5)(q31), i(5p), add
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-208-144-6005
