HN

Cat.No.: CSC-C0458

Species: Homo sapiens ,human

Source: oral squamous cell carcinoma

Morphology: epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogeneous) growing in multilayers

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Cat.No.

CSC-C0458

Description

Established from the tumor of a 60-year-old Japanese man with moderately differentiated squamous cell carcinoma of the soft palate which was invasive into muscle layer; cell line was established from a cervical lymph node metastasis which occurred seven years after initial treatment of the primary tumor; the tumor metastasized not only to the lungs, but also to the brain; described as anchorage-independent and tumorigenic in nude mice

Species

Homo sapiens ,human

Source

oral squamous cell carcinoma

Recommended Medium

90% DMEM + 10% h.i.FBS

Morphology

epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogeneous) growing in multilayers

STR DNA Profile

Amelogenin: X,X
CSF1PO: 11
D13S317: 10
D16S539: 12,13
D18S51: 13
D21S11: 29,30
D3S1358: 15
D5S818: 12
D7S820: 10
D8S1179: 13
FGA: 23
Penta D: 10,13
Penta E: 16
TH01: 9
TPOX: 9,11
Vwa: 17

Karyotype

Human flat-moded hypotriploid karyotype with 4% polyploidy - 60-64<3n>XX, -Y, +1, -2, -4, +5, -6, +7, -10, -12, -13, -13, -17, -18, -18, -21, -22, +3-5mar, add(X)(p22), der(1)add(1)(p36)ins(1;?)(q11;?), t(4;14)(q21;p11), i(4p)x1-2, del(5)(q31), i(5p), add

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How stable is L-glutamine in the medium?

The stability of L-glutamine in culture medium is primarily dependent on the temperature at which it is stored. The L-glutamine concentration is reduced by approximately 2-3% per month in tissue culture medium stored at 2-8°C.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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The tumor cell products at Creative Bioarray have been instrumental in our investigations of the tumor microenvironment.

09 Aug 2023

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