Description
Subclone of ACH1P which was established by fusion of the JEG-3-derived HGPRT-defective mutant cell line AC1-1 with primary male trophoblast cells isolated from term human chorion laeve; described as possessing both, a distinct karyotype and a distinct expression pattern of tumor-related genes
Karyotype
Human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-106<4n>XX, -Y, -3, -4, +7, +9, -13, -18, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32)x2, i(13q), add(15)
Source
choriocarcinoma-trophoblast hybrid
Culture Properties
monolayer
Morphology
epithelial-like adherent cells growing in monolayers
Quality Control
Mycoplasma: contamination was eliminated with sparfloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
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USA: 1-631-626-9181
Europe: 44-207-097-1828
