AC-1M81

Cat.No.: CSC-C0485

Species: Homo sapiens (Human)

Morphology: epithelial-like adherent cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0485

Description

Subclone of ACH1P which was established by fusion of the JEG-3-derived HGPRT-defective mutant cell line AC1-1 with primary male trophoblast cells isolated from term human chorion laeve; described as possessing both, a distinct karyotype and a distinct expression pattern of tumor-related genes

Species

Homo sapiens (Human)

Recommended Medium

Ham's F-12 and 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epithelial-like adherent cells growing in monolayers

Karyotype

Human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-106<4n>XX, -Y, -3, -4, +7, +9, -13, -18, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32)x2, i(13q), add(15)

Quality Control

Mycoplasma: contamination was eliminated with sparfloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.