Description
Established in 2003 from the meningothelial meningioma (grade I, WHO, #1238-03) attached to the parietal falx of a 68-year-old woman after surgical tumor resection; cells were immortalized by retroviral transduction with human telomerase reverse transcriptase (hTERT)
Karyotype
Human hypodiploid karyotype with 12% polyploidy; 44(43-46)<2n>XX, -22; frequent random chromosome loss; monosomy 22 is recurrent in grade I benign meningiomas; matches published karyotype
Culture Properties
monolayer
Morphology
adherent, epithelial cells growing as monolayers
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
