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SNB-19

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Cat.No.
CSC-C0391
Description
Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)
Species
Human
Source
astrocytoma
Recommended Medium
90%DMEM + 10% h.i.FBS
Culture Properties
monolayer
Morphology
adherent fibroblastic cells growing as monolayer with contact inhibition, occasional giant cells
Karyotype
Human hypotriploid karyotype with 15% polyploidy - 63(58-63)<3n>XXY, +1, +7, -8, -10, -12, -13, -14, -15, -16, -18, -21, -22, +2mar, der(1)del(1)(q23)ins(1;4)(p32;q?23q27), del(1)(q13), del(4)(q23q27), del(4)(q28q35), add(8)(q24), add(11)(p15), der(19)add
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA:
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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CM-1010X

For research use only. Not for any other purpose.

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Q1:
Should DMSO be removed as soon as a frozen tube of cells is thawed and cultured?
A:

Most cell lines (including suspension cells), except for a few cells that are specifically indicated as being sensitive to DMSO, should be placed directly into a corner bottle containing 10-15ml of fresh medium after thawing and then replaced with fresh medium the next day to remove the DMSO, as this will avoid most problems with cell growth or adhesion after thawing.

Average Rating: 5.0    |    1 Scientist has reviewed this product


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“ Wonderful ”
Rating: 4.8
Review: We have always trusted Creative Bioarray's cellular products to contribute to our research.
Review data: 22 Nov 2022.

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