SNB-19

Cat.No.: CSC-C0391

Species: Human

Source: astrocytoma

Morphology: adherent fibroblastic cells growing as monolayer with contact inhibition, occasional giant cells

Culture Properties: monolayer

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Cat.No.

CSC-C0391

Description

Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)

Species

Human

Source

astrocytoma

Recommended Medium

90%DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

adherent fibroblastic cells growing as monolayer with contact inhibition, occasional giant cells

Karyotype

Human hypotriploid karyotype with 15% polyploidy - 63(58-63)<3n>XXY, +1, +7, -8, -10, -12, -13, -14, -15, -16, -18, -21, -22, +2mar, der(1)del(1)(q23)ins(1;4)(p32;q?23q27), del(1)(q13), del(4)(q23q27), del(4)(q28q35), add(8)(q24), add(11)(p15), der(19)add

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Should DMSO be removed as soon as a frozen tube of cells is thawed and cultured?

Most cell lines (including suspension cells), except for a few cells that are specifically indicated as being sensitive to DMSO, should be placed directly into a corner bottle containing 10-15ml of fresh medium after thawing and then replaced with fresh medium the next day to remove the DMSO, as this will avoid most problems with cell growth or adhesion after thawing.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Wonderful

We have always trusted Creative Bioarray's cellular products to contribute to our research.

22 Nov 2022

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For research use only. Not for any other purpose.