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SNB-19

Cat.No.: CSC-C0391

Species: Human

Source: astrocytoma

Morphology: adherent fibroblastic cells growing as monolayer with contact inhibition, occasional giant cells

Culture Properties: monolayer

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Cat.No.
CSC-C0391
Description
Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)
Species
Human
Source
astrocytoma
Recommended Medium
90%DMEM + 10% h.i.FBS
Culture Properties
monolayer
Morphology
adherent fibroblastic cells growing as monolayer with contact inhibition, occasional giant cells
Karyotype
Human hypotriploid karyotype with 15% polyploidy - 63(58-63)<3n>XXY, +1, +7, -8, -10, -12, -13, -14, -15, -16, -18, -21, -22, +2mar, der(1)del(1)(q23)ins(1;4)(p32;q?23q27), del(1)(q13), del(4)(q23q27), del(4)(q28q35), add(8)(q24), add(11)(p15), der(19)add
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA:
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

SNB-19 cell line – A glioma cell line isolated from a meningioma. They look epithelial- or fibroblast-like in shape and stick most strongly to dishes or flasks in monolayers. Not only does this cell line have wild-type p53 protein, it also has MGMT (O6-methylguanine-DNA methyltransferase), glial marker protein GFAP (glial fibrillary acidic protein), stem cell marker CD133 and high EGFR (epidermal growth factor receptor). In addition, SNB-19 cells also lack the CDKN2A/p16 gene and mutate the PTEN gene, but retain the wild-type IDH1 (isocitrate dehydrogenase 1) gene.

SNB-19 cells possess invasive and migratory capabilities, capable of forming neurospheres in vitro exhibiting stem cell-like properties. These features make them invaluable in studying glioma invasion, migration mechanisms, and stem cell characteristics. Additionally, SNB-19 cells secrete plasminogen activator, an enzyme crucial in the invasion and migration processes of tumor cells. In nude mouse experiments, SNB-19 cells can form tumors, validating their tumorigenic properties. In scientific research, the SNB-19 cell line is widely used to investigate the biological characteristics of gliomas, including tumor invasiveness, migration, and responses to chemotherapeutic agents.

Microscopic observations of SNB-19 cells following 24 and 72 hours of culture.Fig. 1. Microscopic images of SNB-19 after 24h and 72h of culture (Cierluk, K., Szlasa, W., et al., 2020).

Cepharanthine Induces ROS Stress in Glioma and Neuronal Cells Via Modulation of VDAC Permeability

Cepharanthine (CEP) is a bisbenzylisoquinoline alkaloid that may influence the occurrence and development of glioblastoma multiforme (GBM) through Voltage-dependent anion channels (VDAC). Cierluk's team investigated the biological effects of CEP on the glioblastoma cell line (SNB-19) and neuron cell line (PC-12 + NGF), finding that CEP induces ROS stress in glioma and neuronal cells by modulating VDAC permeability. Immunocytochemical staining showed that T-type calcium channel expression in SNB-19 cells increased with higher CEP concentrations, peaking at 10 μM. CEP did not affect T-type channel expression in PC-12 + NGF cells. For VDAC, SNB-19 cells showed a steady increase in expression with higher CEP concentrations, but no time-dependent changes. PC-12 + NGF cells had higher VDAC expression levels at higher CEP concentrations compared to SNB-19 cells, and their initial VDAC expression was significantly higher (Fig. 1). Cells incubated with increasing CEP concentrations showed a rise in ROS levels, especially in SNB-19 cells (Fig. 2A). Over time, even lower CEP concentrations led to greater ROS generation. Upon CEP addition, mitochondrial content dispersed evenly in the cytoplasm compared to the concentrated pattern in controls. In PC-12+NGF cells, ROS release was significant after 24h incubation at lower CEP concentrations, showing a distribution pattern similar to SNB-19 cells (Fig. 2B).

The changes of calcium T-type channel (A) and VDAC (B) were detected by immunocytochemistry ABC method after 24 and 72 h of CEP treatment.Fig. 1. The immunocytochemical evaluation of calcium T-type channel (A) and VDAC (B) with ABC technique after 24 h and 72 h incubation with varying concentrations of CEP (Cierluk, K., Szlasa, W., et al., 2020).

Immunofluorescence staining of ROS in SNB-19 cells (A) and PC-12 + NGF cells (B) after incubation with different concentrations of CEP for (A) 24 h and (B) 72 h.Fig. 2. Immunofluorescent staining of ROS in SNB-19 cells (A) and PC-12 + NGF cells (B) after (a) 24 h and (b) 72 h of incubation with varying CEP concentrations (Cierluk, K., Szlasa, W., et al., 2020).

ZIKV Infection Reduced Mitochondrial Transmembrane Potential

Zika virus (ZIKV) causes neurodevelopmental disruption in newborn babies. Yang's team had studied how mitochondrial shape and function were altered in human NSCs and the human glioblastoma cell line SNB-19 following ZIKV infection. They explained how ZIKV infection disturbs mitochondrial dynamics, network organisation and activity by depleting MFN2 protein. In order to examine mitochondrial fragmentation under ZIKV infection, they speculated that dysregulation could be caused by a mismatch between mitochondrial fusion and fission proteins. In ZIKV-infected NSCs and SNB-19 cells, MFN2 protein levels fell after 24 hours even though infection was only 50 % (Fig. 3A-C). MFN1 reduction occurred in SNB-19 cells but not in NSCs, possibly due to lower neuronal expression (Fig. 3B). Immunofluorescence confirmed weaker MFN2 signals in infected cells compared to uninfected ones (Fig. 3D and E). Fragmented mitochondria were observed in ZIKV ENV-positive cells, indicating reduced mitochondrial fusion (Fig. 3F-H). Protein levels of OPA1, DNM1L, and FIS1 were unchanged, but DNM1L phosphorylation at Ser616, which promotes fission, was absent (Fig. 3B). This dephosphorylation could be a response to excessive fragmentation to curb further fission. Overall, ZIKV infection disrupted the balance of mitochondrial fusion and fission, leading to network fragmentation.

MFN2 protein level was reduced in ZIKV-infected cells.Fig. 3. MFN2 protein level was reduced in ZIKV-infected cells (Yang S, Gorshkov K, et al., 2020).

Should DMSO be removed as soon as a frozen tube of cells is thawed and cultured?

Most cell lines (including suspension cells), except for a few cells that are specifically indicated as being sensitive to DMSO, should be placed directly into a corner bottle containing 10-15ml of fresh medium after thawing and then replaced with fresh medium the next day to remove the DMSO, as this will avoid most problems with cell growth or adhesion after thawing.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

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22 Nov 2022


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