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GOS-3

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Cat.No.
CSC-C0452
Description
Established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); described to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation; DNA fingerprinting at Creative Bioarray showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG
Organism
Human
Karyotype
Human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 ty
Source
glioblastoma
Culture Properties
monolayer
Morphology
fibroblastoid, adherent cells growing as monolayer
Recommended Medium
90% DMEM + 10% h.i.FBS
Quality Control
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA: rev
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-C0317
CSC-C0391
CSC-C9434L
CSC-C8781H
CSC-C8881H
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