GOS-3

Cat.No.: CSC-C0452

Species: Human

Source: glioblastoma

Morphology: fibroblastoid, adherent cells growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0452

Description

Established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); described to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation; DNA fingerprinting at Creative Bioarray showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG

Species

Human

Source

glioblastoma

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

fibroblastoid, adherent cells growing as monolayer

Karyotype

Human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 ty

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA: rev

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How do I freeze the cells?

Collect the cells by centrifugation, remove the supernatant thoroughly from the centrifuge tube, add the appropriate amount of serum-free cell lyophilisation solution to the tube to make a cell lyophilisation suspension and place in a -80°C refrigerator for long-term freezing or transfer to liquid nitrogen

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Ideal products

This product is ideal for studying cell migration due to cell-cell interaction with the extracellular matrix.

05 Oct 2021

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For research use only. Not for any other purpose.