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LN-405
- Specification
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Cat.No.
CSC-C0317
Description
Glioblastoma cells established from an astrocytoma tumor (grade IV) of a 62-year-old woman in 1986; cells were described as expressing mRNA for both TGF-β1 and G-TSF/TGF-β2 (but only the latter polypeptide was secreted) and expressing GFAP protein
Species
Human
Source
astrocytoma
Culture Properties
monolayer
Morphology
fibroblastic, grows as monolayers in culture
Karyotype
Human hypertetraploid karyotype with 37% polyploidy and with 1-3 supernumerary dm present in most cells - 102-108<4n>XXXX, +1, +2, +3, +4, +5, +7, -10, +11, +13, -16, -16, -16, +19, +20, +20, +21, +22, +8mar, del(1)(q41)x2, der(1)t(1;3)(p32;p11), add(4)(p
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What to look for when handling cells for the first time?
A:
Open the cap of the bottle in the ultra-clean bench and wipe the outside of the mouth of the bottle with a cotton ball soaked in 75% alcohol to prevent contamination of the cells with spilled medium.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Perfect
These cells are of high quality and allow reproducible results up to numerous passages.
02 Dec 2020
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