LN-405

Cat.No.: CSC-C0317

Species: Human

Source: astrocytoma

Morphology: fibroblastic, grows as monolayers in culture

Culture Properties: monolayer

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Cat.No.

CSC-C0317

Description

Glioblastoma cells established from an astrocytoma tumor (grade IV) of a 62-year-old woman in 1986; cells were described as expressing mRNA for both TGF-β1 and G-TSF/TGF-β2 (but only the latter polypeptide was secreted) and expressing GFAP protein

Species

Human

Source

astrocytoma

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

fibroblastic, grows as monolayers in culture

Karyotype

Human hypertetraploid karyotype with 37% polyploidy and with 1-3 supernumerary dm present in most cells - 102-108<4n>XXXX, +1, +2, +3, +4, +5, +7, -10, +11, +13, -16, -16, -16, +19, +20, +20, +21, +22, +8mar, del(1)(q41)x2, der(1)t(1;3)(p32;p11), add(4)(p

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What to look for when handling cells for the first time?

Open the cap of the bottle in the ultra-clean bench and wipe the outside of the mouth of the bottle with a cotton ball soaked in 75% alcohol to prevent contamination of the cells with spilled medium.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Perfect

These cells are of high quality and allow reproducible results up to numerous passages.

02 Dec 2020

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For research use only. Not for any other purpose.