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RV-C2
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Cat.No.
CSC-C6166X
Description
Described as secreting a mouse monoclonal antibody (IgG2b) reacting with fetal, neonatal and adult cardiac fibers in immunofluorescence and with rat cardiac fetal and adult troponin T in Western blotting; in immunofluorescence this antibody stains some rat fetal and neonatal skeletal muscle fibers which express fetal cardiac troponin T as also shown by the Western blotting of rat fetal and neonatal skeletal muscle; this cell line was produced by fusing NSO myeloma cells with spleen cells from a Balb/c mouse that had been immunized with a myofibrillar preparation from hypothyroid rabbit ventricle
Species
Mouse
Source
hybridoma
Recommended Medium
80% DMEM + 20% horse serum.
Culture Properties
suspension
Morphology
round single cells in suspension
Karyotype
Murine hypertetraploid/pentaploid karyotype - 88-99<4n> - 2 centric fusion markers and 1-2 dmin present in most cells
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG2b monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Immunology: secretion of a IgG2b monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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