An 8-bromodeoxyuridine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). B82 lacks thymidine kinase and was isolated by virtue of resistance to 8-bromodeoxyuridine (30 µg/ml).
Tissue of Origin
L cell fibroblast
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine
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