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RH-30

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Cat.No.
CSC-C0500
Description
Established from the bone marrow metastasis of a 17-year-old man with undifferentiated (standard classification) or unclassified (Palmer classification) alveolar rhabdomyosarcoma; cells were described as carrying a p53 mutation, expressing high levels of both myogenin and myoD, and expressing the Pax3/FKHR fusion protein secondary to the t(2;13)(q35;q14) translocation
Organism
Human
Karyotype
Human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q)
Source
rhabdomyosarcoma
Culture Properties
monolayer
Morphology
adherent epithelial-like cells growing as monolayer
Recommended Medium
90-95% RPMI-1640 + 5-10% h.i. FBS
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, desmin +, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6258W
CSC-6266W
CSC-C0362
CSC-C0367
CSC-C0473
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