Established from the tumor of a 22-year-old man with metastatic Ewings sarcoma that arose in the humerus; cell line was derived in 1981 from a biopsy of recurrent tumor at the primary site
Human flat-moded hypertriploid karyotype with 4% polyploidy - 70-80<3n>XX, -Y, +1, +2, -3, +6, +8, -9, +11, -17, +21, +2-11 mar, add(3)(q29), add(6)(q25)x2-3, del(8)(p22), add(8)(p22), del(9(p24), t(11;22)(q24;q12)x2, i(13q)x1-2, add(14)(p11) - sideline w
loosely adherent cells growing in monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament +, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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