Established from the recurrence of a dedifferentiated chondrosarcoma (grade III) of the muscle of the thigh from a 76-year-old man in 1989; tumorigenic chondrosarcomatous differentiation of xenografted tumor
adherent fibroblast-like cells growing as monolayer; the culture contains a large amount of cellular debris
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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