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PB-1
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Cat.No.
CSC-C2043
Description
Established from the bone marrow of a CBA/C57BL mouse in 1995
Species
Mouse
Source
pre B cells
Recommended Medium
85% SuperCult® McCoys 5a Medium + 15% h.i. FBS + 50 ng/ml Human IL-7 + 0.4x MEM vitamins + 1x MEM non-essential amino acids + 0.5x essential amino acids + 1 mM sodium pyruvate + 0.12% sodium bicarbonate + 2 mM L-glutamine + 50 μM mercaptoethanol
Culture Properties
suspension
Morphology
cells are very small and grow mainly in small clusters, but also singly in suspension
Karyotype
Murine near-diploid karyotype
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: IL-7 dependency confirmed by 3H-thymidine assay
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Immunology: IL-7 dependency confirmed by 3H-thymidine assay
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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