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N3-36

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Cat.No.
CSC-C6184X
Description
Described as secreting a mouse monoclonal antibody (IgM) reacting with neonatal and adult fast fibers and myosins in rodent and human on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Organism
Mouse
Karyotype
Cytogenetics: murine hypertetraploid karyotype, 20% polyploidy - 78-90 - carries large telocentric and two centric fusion markers
Source
hybridoma (anti-fiber/myosin)
Morphology
single round cells, lightly adherent
Recommended Medium
90% DMEM + 10% h.i.FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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