D3

Cat.No.: CSC-C6186X

Species: Mouse

Source: hybridoma (anti-chicken N-CAM)

Morphology: round, single cells growing in suspension and some slightly adherent

Culture Properties: suspension

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Cat.No.

CSC-C6186X

Description

Cell line was established by fusion of mouse NS-1 cells with spleen cells from a BALB/c mouse immunized with wheat germ agglutinin affinity purified chicken brain proteins immobilized on lectin beads; described as secreting a mouse monoclonal antibody (IgG) reacting with the cytoplasmic domain of the 140, 180 and 250 kDa glycosylated neuronal cell surface protein N-CAM, preferentially in neurons of the chicken visual system like retina, tectum and other CNS regions and PNS; also reacting with mouse, goldfish and human N-CAM in paraformaldehyde-fixed cryosections and Western blots

Species

Mouse

Source

hybridoma (anti-chicken N-CAM)

Recommended Medium

80% RPMI-1640 I-1640 + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement")

Culture Properties

suspension

Morphology

round, single cells growing in suspension and some slightly adherent

Karyotype

Murine flat-moded hypertetraploid karyotype - 98-104<4n> - two centric fusion markers present

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgG2A monoclonal antibody
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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