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C7
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Cat.No.
CSC-C6195X
Description
Established from the bone marrow of male adult 129/Sv mice (p53 gene knocked out); it is described that the differentiation potential of C7 cells to osteoclasts is maintained in the presence of M-CSF and is abolished irreversibly by the stimuli known to induce differentiation into other hematopoietic lineages
Species
Mouse
Source
bone marrow
Recommended Medium
SuperCult® AlphaMEM + 10% h.i. FBS + 1 ng/ml M-CSF (Human or murine)
Morphology
macrophage-like adherent cells with processes
Karyotype
Murine hypertriploid karyotype with 20% polyploidy - 66(64-67)<3n> - no centric fusion markers present
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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