An immortalised mouse neural progenitor cell line capable of differentiation in vitro. The cell line was established by retorviral-mediated transduction of the avian myc oncogene into mitotic progenitor cells of neonatal mouse cerrebellum. Mouse strain CD1 x C57BL/6. This cell line is a valuable tool for in vitro and in vivo studies aimed at understanding the control of cell fate and differentiation of neural progenitors. The MMLV retrovirus vector used for the immortalisation process contained a neo resistance gene transcribed from an internal SV40 promoter. Therefore the cells are neo resistant. The morphology of the cells may change over time. Cells plated at low density may tend to become more process bearing whereas those plated more densely may tend to become flat and non-process bearing.
Tissue of Origin
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Flasks should be pre-coated with poly-L-lysine at 10µg/ml in sterile distilled water.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Liquid Nitrogen (-180 °C).
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