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BF-G6
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Cat.No.
CSC-C6169X
Description
Described as secreting a mouse monoclonal antibody (IgG1) reacting with human and rat embryonic fibers in immunofluorescence and with human and rat embryonic myosin heavy chains (MHCs) in Western blotting; this antibody reacts more weakly also with adult rat 2B fibers in immunofluorescence and adult rat 2B MHCs in Western blotting; moreover, it stains muscle cells in the conduction tissue of newborn rat heart; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine fetal muscles
Species
Mouse
Source
hybridoma (anti-rat fiber/myosin)
Recommended Medium
90% DMEM + 10% horse serum.
Culture Properties
suspension
Morphology
single round cells in suspension (sometimes with loosely adherent cells, sometimes in clumps)
Karyotype
Murine hypertetraploid karyotype - 82(79-88)<4n> - 2-3 dm present in most cells
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assaysImmunology:secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 3-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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