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BF-32
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Cat.No.
CSC-C6176X
Description
Described as secreting a mouse monoclonal antibody (IgM) reacting with type I and IIA fibers in immunofluorescence and with type 1 and 2A myosin heavy chains (MHCs) in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine fetal muscles
Species
Mouse
Source
hybridoma (anti-rat fiber/myosin)
Recommended Medium
90% DMEM + 10% horse serum.
Culture Properties
suspension
Morphology
grows as single cells or in clumps in suspension; loosely adherent at times
Karyotype
Murine hypotetraploid karyotype with 4.5% polyploidy - 72-80<4n> - 2-4 dms present in most cells
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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