IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.
Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>
mostly single, round (some polymorph) cells in suspension (or occasionally in clumps)
+ 10% h.i. FBS
+ 10 ng/ml IL-3 or 10% vol conditioned medium of WEHI-3B cell line
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD11b (+), F4/80 (+)
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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