BA/F3

Specification
Technical Resources
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Cat.No.

CSC-C2045

Description

IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.

Source

pro B cells

Recommended Medium

90%RPMI-1640 + 10% h.i. FBS + 10 ng/ml IL-3 or 10% vol conditioned medium of WEHI-3B cell line

Culture Properties

suspension

Morphology

mostly single, round (some polymorph) cells in suspension (or occasionally in clumps)

Karyotype

Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>

Markers

CD11b (+), F4/80 (+)

Reference

Publication

Applications

a. Kinase drug discovery. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
b. Cell-Based assay
c. In vivo efficacy study

Quality Control

Mycoplasma: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Selective biochemical test: negative
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

Storage and Shipping

Ship in dry ice.
Store in liquid nitrogen.

BioSafety Level

BSL–1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.,

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Product Sheets

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