BA/F3

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BA/F3

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Cat.No.
CSC-C2045
Description
IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.
Organism
Mouse
Source
pro B cells
Recommended Medium
90%RPMI-1640 + 10% h.i. FBS + 10 ng/ml IL-3 or 10% vol conditioned medium of WEHI-3B cell line
Culture Properties
suspension
Morphology
mostly single, round (some polymorph) cells in suspension (or occasionally in clumps)
Karyotype
Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>
Markers
CD11b (+), F4/80 (+)
Reference
Applications
a. Kinase drug discovery. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
b. Cell-Based assay
c. In vivo efficacy study
Quality Control
Mycoplasma: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Selective biochemical test: negative
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Storage and Shipping
Ship in dry ice.
Store in liquid nitrogen.
BioSafety Level
BSL–1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.,
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Product Sheets

PDF Datasheet



No 'Material Safety Data Sheet' available yet

Q1:
Can you provide the QC of the cell line? For example, mycoplasma test? Do you have the genomic data to make sure there's no contamination.
A:

Yes, of course. We can provide you with the COA of BA/F3, which includes QC test results of each lot.

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