IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.
+ 10% h.i. FBS
+ 10 ng/ml IL-3 or 10% vol conditioned medium of WEHI-3B cell line
mostly single, round (some polymorph) cells in suspension (or occasionally in clumps)
Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>
CD11b (+), F4/80 (+)
a. Kinase drug discovery. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
b. Cell-Based assay
c. In vivo efficacy study
Mycoplasma: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Selective biochemical test: negative
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Storage and Shipping
Ship in dry ice.
Store in liquid nitrogen.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.,
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No 'Material Safety Data Sheet' available yet