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BA/F3

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Cat.No.
CSC-C2045
Description
IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.
Organism
Mouse
Karyotype
Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>
Source
pro B cells
Culture Properties
suspension
Morphology
mostly single, round (some polymorph) cells in suspension (or occasionally in clumps)
Recommended Medium
90%RPMI-1640 + 10% h.i. FBS + 10 ng/ml IL-3 or 10% vol conditioned medium of WEHI-3B cell line
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD11b (+), F4/80 (+)
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Reference
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Product Sheets

PDF Datasheet



No 'Material Safety Data Sheet' available yet

Q1:
Can you provide the QC of the cell line? For example, mycoplasma test? Do you have the genomic data to make sure there's no contamination.
A:

Yes, of course. We can provide you with the COA of BA/F3, which includes QC test results of each lot.

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Epidermal growth factor receptor mutation mediates cross-resistance to panitumumab and cetuximab in gastrointestinal cancer Authors: Braig F, März M, Schieferdecker A, et al.
The Oncotarget. 2015, 6(14): 12035.
Cetuximab Resistance in Head and Neck Cancer Is Mediated by EGFR-K 521 Polymorphism Authors: Friederike Braig, Malte Kriegs, Minna Voigtlaender, Beate Habel, Tobias Grob, Karina Biskup, Veronique Blanchard, Markus Sack, Anja Thalhammer, Isabel Ben Batalla, Ingke Braren, Simon Laban, Antje Danielczyk, Steffen Goletz, Elzbie...
Cancer Research, 2017, 77(5): 1188-1199.
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