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BA/F3

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Cat.No.
CSC-C2045
Description
IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.
Organism
Mouse
Karyotype
Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>
Source
pro B cells
Culture Properties
suspension
Morphology
mostly single, round (some polymorph) cells in suspension (or occasionally in clumps)
Recommended Medium
90%RPMI-1640 + 10% h.i. FBS + 10 ng/ml IL-3 or 10% vol conditioned medium of WEHI-3B cell line
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD11b (+), F4/80 (+)
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Reference
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Product Sheets

PDF Datasheet



No 'Material Safety Data Sheet' available yet

Q1:
Can you provide the QC of the cell line? For example, mycoplasma test? Do you have the genomic data to make sure there's no contamination.
A:

Yes, of course. We can provide you with the COA of BA/F3, which includes QC test results of each lot.

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Epidermal growth factor receptor mutation mediates cross-resistance to panitumumab and cetuximab in gastrointestinal cancer Authors: Braig F, März M, Schieferdecker A, et al.
The Oncotarget. 2015, 6(14): 12035.
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CSC-6287W
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