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BA-D5

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Cat.No.
CSC-C6170X
Description
This hybridoma cell line was described to secrete a mouse monoclonal antibody (IgG) that reacts with rat type 1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain) in western blotting; the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin from bovine atrium
Organism
Mouse
Karyotype
Murine hypertetraploid/pentaploid karyotype with 10% polyploidy - 90(88-100)<4n> - 1-3 centric fusion markers and 2 dmin present in most cells
Source
hybridoma (anti-rat fiber/myosin)
Culture Properties
suspension
Morphology
single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells)
Recommended Medium
90% DMEM + 10% h.i.FBS (or horse serum.)
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG2b monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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