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Cat.No.
CSC-C0620
Description
Established in 2002 from the mononuclear ascites cells of a 57-year-old man with terminal diffuse large B cell lymphoma (stage IV) after failing to respond to several rounds of chemotherapy (CHOP, ICE)
Organism
Human
Karyotype
Human hyperdiploid karyotype with 8% polyploidy 47(42-48)<2n>XY, +7, +8, -15, der(3)del(3)(p21p22)t(3;8)(q27;q24.1), t(3;8)(q27;q24.3), t(4;20)(q21;p12), t(7;12)(q22;p13), der(8)t(3;8)(q27;q24.3), der(8)t(3;8)(q27;q23)t(8;7)(q24.3;q11), inv(9)(p21q34), de
Source
B cell lymphoma
Morphology
round cells growing adherently as single cells or in small clumps when cultured on extracellular matrix (Matrigel)
Recommended Medium
80% SuperCult® IMDM+ EMEM (at 1:1) + 20% h.i. FBS
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
Viruses: PCR: EBV -, HBV -, HC
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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