ULA
Cat.No.: CSC-C0620
Species: Homo sapiens (Human)
Source: Ascites
Morphology: round cells growing adherently as single cells or in small clumps when cultured on extracellular matrix (Matrigel)
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Cat.No.
CSC-C0620
Description
Established in 2002 from the mononuclear ascites cells of a 57-year-old man with terminal diffuse large B cell lymphoma (stage IV) after failing to respond to several rounds of chemotherapy (CHOP, ICE)
Species
Homo sapiens (Human)
Source
Ascites
Recommended Medium
Morphology
round cells growing adherently as single cells or in small clumps when cultured on extracellular matrix (Matrigel)
Karyotype
Human hyperdiploid karyotype with 8% polyploidy 47(42-48)<2n>XY, +7, +8, -15, der(3)del(3)(p21p22)t(3;8)(q27;q24.1), t(3;8)(q27;q24.3), t(4;20)(q21;p12), t(7;12)(q22;p13), der(8)t(3;8)(q27;q24.3), der(8)t(3;8)(q27;q23)t(8;7)(q24.3;q11), inv(9)(p21q34), de
Disease
Diffuse Large B-Cell Lymphoma
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
Viruses: PCR: EBV -, HBV -, HC
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
Viruses: PCR: EBV -, HBV -, HC
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Synonyms
Uppsala Lymphoma Ascites-fluid
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