UCSD-AML1

Specification
Recommended Products

Cat.No.

CSC-C0672

Description

Established from the bone marrow of a 73-year-old woman with acute myeloid leukemia at relapse in 1989; cells were described to be strictly cytokine-dependent (GM-CSF, IL-6, or M-CSF) and to carry the translocations t(3;3)(q21;q26) and t(12;22)(p13;q12) leading to MECOM (EVI1) overexpression and to ETV6-MN1 (TEL-MN1) fusion gene, respectively

Species

Human

Source

acute myeloid leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS + 10 ng/ml GM-CSF

Culture Properties

suspension

Morphology

single cells in suspension, a few cells are lightly adherent

Karyotype

Human flat-moded hypodiploid karyotype with 10% polyploidy; 45(42-45)<2n>XX,-7, t(3;3)(q21;q26), t(12;22)(p13;q12); carries t(3;3) and t(12;22) effecting MN1 and ETV6/MN1 rearrangements, respectively; precisely matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

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CS-001Y

PremSera® FBS

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For research use only. Not for any other purpose.

Q & A
Customer Review

How are STRs detected by PCR?

Historically, STRs have been genotyped using polymerase chain reaction (PCR) and gel electrophoresis. In such cases, PCR is performed using primers complementary to unique sequences flanking the STR. The PCR product is then run on a capillary electrophoresis gel to determine its size.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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The products are shipped quickly and securely, with careful packaging to prevent damage or contamination during transit.

30 June 2023

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