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SU-DHL-4
Cat.No.: CSC-C0506
Species: Human
Source: peritoneal effusion
Morphology: single round cells growing in suspension
Culture Properties: suspension
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Cell type: B cell lymphoma
Origin: established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell)
D13S317: 11, 12
D7S820: 8, 11
D16S539: 11, 13
vWA: 18, 19
THO1: 6, 9.3
Amelogenin: X Y
TPOX: 9, 11
CSF1PO: 12
SU-DHL-4 is a lymphoblast-like cell that was isolated from the peritoneal effusion of a White, 38-year-old, male patient. This cell line represents a model of diffuse large B-cell lymphoma (DLBCL), one of the most common types of non-Hodgkin lymphoma in adults. The establishment of this cell line has provided valuable insights into the biology of DLBCL, especially concerning the cellular and molecular mechanisms underlying lymphomagenesis and tumor progression.
SU-DHL-4 cells have been widely used to study the efficacy and mechanism of action of various chemotherapeutic and targeted therapeutic agents, reflecting their importance in lymphoma treatment research. The cells express several key immunophenotypic markers associated with the B-cell lineage such as CD19 and CD20, which are essential for the development and function of B-lymphocytes. These markers also make SU-DHL-4 an excellent target for testing B-cell-specific therapies, including monoclonal antibodies and small molecule inhibitors that disrupt key signaling pathways involved in lymphoma cell survival and proliferation.
APOC1 Knockdown Induces Apoptosis and Decreases Angiogenesis in Diffuse Large B-Cell Lymphoma Cells
Apolipoprotein C1 (APOC1) plays a significant role in the proliferation and metastasis of various malignant tumors; however, its role in diffuse large B-cell lymphoma (DLBCL)—particularly its effects on angiogenesis—remains largely unexplored. This study investigates the functional role of APOC1 in DLBCL development and its potential molecular mechanisms through in vitro and in vivo experiments. Results showed that APOC1 is overexpressed in DLBCL tissues and cells, with high APOC1 levels associated with poor patient prognosis. In vitro experiments revealed that APOC1 knockdown increased apoptosis and inhibited cell proliferation, migration, invasion, HUVEC angiogenesis, and PI3K/AKT/mTOR signaling pathway protein expression in DLBCL cells.
Fig. 1. Knockdown of APOC1 decreases DLBCL cellular carcinogenic activities (Gao, Jing, et al. 2025).
Fig. 2. Knockdown of APOC1 reduces angiogenesis in HUVECs (Gao, Jing, et al. 2025).
Chidamide and Orelabrutinib Synergistically Induce Cell Cycle Arrest and Apoptosis in Diffuse Large B-Cell Lymphoma
In vitro experiments were conducted using DB and SU-DHL-4 cells treated with chidamide, orelabrutinib, and a combination of both. Cell viability was assessed by cell counting kit-8. Cell apoptosis and the cell cycle were determined using flow cytometry. Reactive oxygen species (ROS) production and mitochondrial function were assessed through ROS and JC-1 staining. RNA sequencing and western blot analyses were conducted to elucidate the molecular mechanisms underlying the combined action of chidamide and orelabrutinib in diffuse large B-cell lymphoma (DLBCL) cells.
This investigation revealed markedly enhanced antiproliferative effects when chidamide was combined with orelabrutinib. The findings of this study provide a compelling justification for the clinical utilization of chidamide and orelabrutinib to treat relapsed/refractory DLBCL.
Fig. 3. Effect of chidamide and orelabrutinib in DLBCL cell line apoptosis (Wu, Chunyan, et al. 2024).
Fig. 4. Effects of chidamide and orelabrutinib on c-Myc, BCL2, TP53, and other apoptosis proteins in DLBCL cells (Wu, Chunyan, et al. 2024).
The main purpose of staining is to highlight the specimen by increasing the contrast between the organisms and the background. Some common examples of stains are methylene blue, crystal violet, carbon fuchsin, etc. Hence, staining improves the observations of any particular cell or tissue when seen under a microscope.
SU-DHL-4 cells are assigned to the GCB-like (germinal center B-cell-like) lymphoma subtype.
Yes, SU-DHL-4 cells are widely used in research studies related to B-cell lymphomas, particularly in investigations involving the EZH2 mutation and GCB-like lymphoma subtype.
Yes, SU-DHL-4 cells are frequently used as a model for studying drug sensitivity and testing potential therapies for diffuse large B-cell lymphoma, especially in the context of EZH2 mutations and GCB-like subtypes.
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Average Rating: 5.0 | 3 Scientist has reviewed this product
Valuable
Tumor cell products are a valuable resource for researchers studying cancer, and Creative Bioarray's products bring great convenience to my experiments.
04 Jan 2022
Ease of use
After sales services
Value for money
Excellent growth characteristics
The SU-DHL-4 cells have exhibited excellent growth characteristics and maintained stability throughout our experiments, which has allowed for reliable and reproducible results.
13 Sep 2023
Ease of use
After sales services
Value for money
Technical assistance
Throughout the process, Creative Bioarray has been incredibly responsive and supportive. They promptly answered my queries and provided technical assistance when needed.
11 May 2023
Ease of use
After sales services
Value for money
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