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RPMI-8402

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Cat.No.
CSC-C0376
Description
Established from the peripheral blood of a 16-year-old woman with acute lymphoblastic leukemia (ALL) in 1972
Organism
Human
Karyotype
Human hypotetraploid karyotype with 2% polyploidy - 90(79-91)<4n>XXX, -X, +3, +3, -10, -13, -14, +15, -18, -20, +2mar, dup(4)(q13q23)x2, del(6)(q14q22)x2, t(11;14)(p15;q11)x2, add(15)(p13) - sideline with der(1)t(1;9)(p35/36;q11), add(13)(q34) - carries t
Source
T cell acute lymphoblastic leukemia
Culture Properties
suspenson
Morphology
round single cells in suspenson, growing partly in clumps
Recommended Medium
Quality Control
Mycoplasma: contaminaton was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 +, CD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRg
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6249W
CSC-6250W
CSC-6280W
CSC-6281W
CSC-6282W
CSC-6296W
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