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RPMI-8402
- Specification
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Cat.No.
CSC-C0376
Description
Established from the peripheral blood of a 16-year-old woman with acute lymphoblastic leukemia (ALL) in 1972
Species
Human
Source
T cell acute lymphoblastic leukemia
Culture Properties
suspenson
Morphology
round single cells in suspenson, growing partly in clumps
Karyotype
Human hypotetraploid karyotype with 2% polyploidy - 90(79-91)<4n>XXX, -X, +3, +3, -10, -13, -14, +15, -18, -20, +2mar, dup(4)(q13q23)x2, del(6)(q14q22)x2, t(11;14)(p15;q11)x2, add(15)(p13) - sideline with der(1)t(1;9)(p35/36;q11), add(13)(q34) - carries t
Quality Control
Mycoplasma: contaminaton was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 +, CD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRg
Immunology: CD2 +, CD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRg
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What is the difference between DMEM and MEM?
A:
The major differences between DMEM and MEM include the use of lower concentrations of amino acids, vitamins, and inorganic phosphate and the inclusion of lactate and glutamine in the medium.
Average Rating: 4.0 | 1 Scientist has reviewed this product
Easy to culture
These cells are easy to culture, which greatly facilitates our experiments.
12 June 2022
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