PA-TU-8988S

Name: PA-TU-8988S
Brand: Creative Bioarray

Cat.No.: CSC-C0326

Species: Homo sapiens (Human)

Source: Liver Metastasis

Morphology: adherent epitheloid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0326

Description

Established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman (sister cell line is PA-TU-8988T)

Species

Homo sapiens (Human)

Source

Liver Metastasis

Recommended Medium

90% DMEM + 5%h.i. FBS + 5%horse serum

Culture Properties

monolayer

Morphology

adherent epitheloid cells growing in monolayers

Karyotype

Human near triploid karyotype with 6% polyploidy - 71(67-77)<3n>XXX, +3, -4, +7, -8, +9, +11, +11, -15, -17, -18, -18, -19, -21, -22, +6mar, del(1)(p21), del(3)(p11p22), del(6)(q26), del(9)(p12), dup(11)(p14->pter), del(12)(p13), add (15)(p12)

Disease

Pancreatic Adenocarcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

PaTu 8988s; PaTu8988s; PaTu8988S; PATU8988S; PaTu 8988 S; PaTu-8988s; PATU-8988S; PATU-S; PA-TU S

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

PA-TU-8988S (also designated PaTu 8988s) is a well-characterized human pancreatic adenocarcinoma cell line. It was established in 1985 from a liver metastasis of a primary pancreatic adenocarcinoma in a 64-year-old female patient. The cells exhibit adherent epitheloid morphology and grow as monolayers. PA-TU-8988S harbors clinically relevant driver mutations in KRAS, SMAD4, and TP53, mirroring the genetic landscape of human pancreatic ductal adenocarcinoma.

A defining feature of PA-TU-8988S is its origin as one of two sister lines—alongside PA-TU-8988T—derived from the same metastatic lesion. While PA-TU-8988T exhibits a mesenchymal-like, spindle-shaped morphology with high ganglioside levels and low metastatic capacity, PA-TU-8988S displays an epithelial-like phenotype with preserved E-cadherin expression, cell-cell contacts, and polarity. Importantly, PA-TU-8988S is the metastatic counterpart: upon intravenous injection into nude mice, it selectively colonizes the lung, whereas PA-TU-8988T fails to metastasize. This isogenic pair provides an exceptional model for dissecting the molecular determinants of metastasis.

Chamber Gap Assay Enables Sensitive Analysis of Cancer-Induced Macrophage Migration

Pancreatic cancer ranks among the most lethal malignancies. A key contributor to its poor prognosis is the capacity of tumor cells to recruit macrophages and reprogram them into a tumor-promoting rather than tumor-eliminating phenotype. Therefore, elucidating how and why macrophages are recruited to the tumor is essential for designing new therapeutic strategies. However, widely used laboratory methods do not permit direct visualization of cell movement or accurate quantification of migration dynamics. Here, we adapted and evaluated a novel in vitro method, the Chamber Gap Assay (CGA). By culturing cancer cells and macrophages in separate but connected compartments, this system permits real-time visualization of macrophage migration toward cancer cells and the resulting morphological changes.

In the model assessing the effects of human pancreatic cancer cell lines PA-TU-8988T and PA-TU-8988S on the migration of THP-1 macrophages, we observed a time-dependent increase in THP-1 macrophage migration (Fig. 1A,B), an increased migration toward pancreatic cancer cells (Fig. 1C), and a gradual rise in the number of migrated THP-1 cells, including cells reaching migration distances beyond 500 µm (Fig. 1D).

Chamber Gap Assay (CGA) enables direct visualization and quantification of THP-1 macrophage migration toward pancreatic cancer cells PA-TU-8988T and PA-TU-8988S. — Fig. 1. Microscopic pictures of migrated THP-1 cells and quantification of migration in CGA (Lenz, Maik, *et al.*, 2026).

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For research use only. Not for any other purpose.