MT-3

Cat.No.: CSC-C0448

Species: Homo sapiens (Human)

Source: Intestine; Colon

Morphology: epithelial cells, after splitting cells start to grow in colonies (not necessarily as monolayer)

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Cat.No.

CSC-C0448

Description

Originated from surgical material of a human breast carcinoma; the tumor was passaged in nude mice prior to adaptation to continuous growth in culture vessels

Species

Homo sapiens (Human)

Source

Intestine; Colon

Recommended Medium

90% RPMI-1640 + 2 mM L-glutamine + 1 mM sodium pyruvate + 10% h.i. FBS

Morphology

epithelial cells, after splitting cells start to grow in colonies (not necessarily as monolayer)

Karyotype

Human hyperdiploid karyotype with 8% polyploidy - 47(45-48)<2n>X, -X, +7, +22, i(13q), del(22)(q11) - trisomy 7 recurrent in breast cancer - resembles published karyotype

Disease

Colon Adenocarcinoma

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokerati

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.