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MT-3

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Cat.No.
CSC-C0448
Description
Originated from surgical material of a human breast carcinoma; the tumor was passaged in nude mice prior to adaptation to continuous growth in culture vessels
Organism
Human
Karyotype
Human hyperdiploid karyotype with 8% polyploidy - 47(45-48)<2n>X, -X, +7, +22, i(13q), del(22)(q11) - trisomy 7 recurrent in breast cancer - resembles published karyotype
Source
colon carcinoma
Morphology
epithelial cells, after splitting cells start to grow in colonies (not necessarily as monolayer)
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokerati
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6288W
CSC-6289W
CSC-6290W
CSC-6291W
CSC-6292W
CSC-6293W
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