Originated from surgical material of a human breast carcinoma; the tumor was passaged in nude mice prior to adaptation to continuous growth in culture vessels
Human hyperdiploid karyotype with 8% polyploidy - 47(45-48)<2n>X, -X, +7, +22, i(13q), del(22)(q11) - trisomy 7 recurrent in breast cancer - resembles published karyotype
epithelial cells, after splitting cells start to grow in colonies (not necessarily as monolayer)
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokerati
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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