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Cat.No.: CSC-C0001

Species: Human

Source: B cell lymphoma

Morphology: round cells growing singly in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0001

Description

Established from peripheral blood mononuclear cells of a 68-year-old white woman with mantle cell lymphoma at relapse in 1997; described to carry a t(11;14)(q13;q32) leading to CCND1-IGH@ (BCL1-IGH) fusion gene

Species

Human

Source

B cell lymphoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing singly in suspension

Karyotype

Human flat moded hypertriploid karyotype; 70-75<3n>XXY, -3, -5, +7, +14, -16, -16, -18, -19, +20, +20, -21, +4mar, del(3)(q13)x1-2, del(6)(22), i(7)(p10), der(8)t(8;14)(q24;q32)t(14;11)(q32;q13)x2, t(8;14)(q24;q32)x2, der(11)t(11;14)(q2?;q11)t(14;8)(q32;q

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between adherent and non adherent cells?

In biologics, two techniques of growing cells in culture can be distinguished: adherent and suspension. In adherent cell culture, cells are grown while attached to a substrate as monolayers. In suspension cell culture, cells are free floating in the culture medium.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Convenient

Creative Bioarray offers a range of complementary products and services, including media and reagents, to support the successful use of tumor cell products.

10 Nov 2022

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