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MC-116

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Cat.No.
CSC-C0238
Description
Established from the pleural effusion of a patient with B cell lymphoma (undifferentiated lymphoma); cells were described to be EBV-negative, to carry the t(8;14) and to express mRNA of the proto-oncogene BCL2
Tissue of Origin
Ascites
Organism
Homo sapiens (human)
Karyotype
Human near diploid karyotype with 1.5% polyploidy - 46(37-46)<2n>XY, dup(1)(q21q32), t(8;14)(q24;q32), del(10)(q23)
Culture Properties
suspension
Morphology
round to elongated, lymphoblastoid, single or clustered cells in suspension
Recommended Medium
80-85% RPMI-1640 + 15-20% h.i. FBS
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda +
Viruses: ELISA: reverse transcriptase
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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