Established from the pleural fluid of a 50-year-old Japanese woman with ductal carcinoma of the breast with a predominant intraductal component in 1992; cells were described to express estrogen, but not progesteron receptors and to secrete tissue polypeptide antigen; however, DNA fingerprinting analysis at the Creative Bioarray showed cross-contamination with cell line MCF-7; MCF-7 was established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma
Human highly rearranged hypertriploid karyotype with 4% polyploidy - 77(70-82)<3n>XX, -X, -1, +2, -11, -13, +14, +15, -17, -18, -19, -20, -20, -21, -22, +15mar - add(X)(q28), add(2)(q35), add(3)(p25)x2, add(5)(p15), add(7)(p14), add(7)(p14), add(8)(p12),
adherent growing in monolayers (very dense colonies)
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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