KPL-1

Cat.No.: CSC-C0389

Species: Human

Source: breast carcinoma

Morphology: adherent growing in monolayers (very dense colonies)

Culture Properties: monolayer

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Cat.No.

CSC-C0389

Description

Established from the pleural fluid of a 50-year-old Japanese woman with ductal carcinoma of the breast with a predominant intraductal component in 1992; cells were described to express estrogen, but not progesteron receptors and to secrete tissue polypeptide antigen; however, DNA fingerprinting analysis at the Creative Bioarray showed cross-contamination with cell line MCF-7; MCF-7 was established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma

Species

Human

Source

breast carcinoma

Recommended Medium

90% DMEM+ 10% h.i.FBS

Culture Properties

monolayer

Morphology

adherent growing in monolayers (very dense colonies)

Karyotype

Human highly rearranged hypertriploid karyotype with 4% polyploidy - 77(70-82)<3n>XX, -X, -1, +2, -11, -13, +14, +15, -17, -18, -19, -20, -20, -21, -22, +15mar - add(X)(q28), add(2)(q35), add(3)(p25)x2, add(5)(p15), add(7)(p14), add(7)(p14), add(8)(p12),

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Where should incubators be placed to avoid physical contamination?

Incubators should be placed in an environment with a constant temperature and not surrounded by equipment that can cause mechanical vibrations, such as centrifuges.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Professional

The sales staff in the Creative Bioarray were very professional and gave lots of useful advice.

11 Jan 2023

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For research use only. Not for any other purpose.