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KI-JK

Cat.No.: CSC-C0676

Species: Human

Source: anaplastic large cell lymphoma

Morphology: round to polymorph cells growing in suspension

Culture Properties: suspension

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Cat.No.
CSC-C0676
Description
Established in 1989 from the pleural effusion of a 15-year-old boy with Ki-1 positive lymphoma (anaplastic large cell lymphoma; ALCL); carries the translocation t(2;5) leading to NPM1-ALK fusion gene; cells were originally described to be EBV positive; however, viral analyses (PCR and western blot) at the Creative Bioarray established that the cells do not harbor EBV
Species
Human
Source
anaplastic large cell lymphoma
Recommended Medium
80% RPMI-1640 + 20% h.i. FBS
Culture Properties
suspension
Morphology
round to polymorph cells growing in suspension
Karyotype
Human flat-moded hypertriploid karyotype; 75-82 <3n>XXYY, +1, +1, +2, +3, +6, +7, +8, -9, +16, -15, -17, +19, +20, +21, +2-3mar, add(Y)(q11), del(1)(p34)x2, der(1)t(1;5)(p11;q11)t(5;2)(q35;p21), der(1;16)(q10;p10), i(1)(q10)x2, t(2;5)(p23;q35)x1-2, add(4)
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD4 -, CD14 -, CD15 +, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are the three types of cell culture techniques?

Cells cultured in the lab can be classified into three different types, including primary cells, transformed cells, and self-renewing cells.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Excellent

The product arrived in excellent condition, with no signs of damage.

26 Sep 2022


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