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KHYG-1

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Cat.No.
CSC-C0784
Description
Established 1997 from the peripheral blood of a 45-year-old woman with aggressive natural killer cell (NK) leukemia; cells were described to display strong cytotoxic activity and to carry a point mutation in exon 7 of the TP53 (p53) gene
Organism
Human
Source
natural killer cell leukemia
Recommended Medium
90% RPMI-1640 + 10% h.i. FBS+ 10 ng/ml IL-2
Culture Properties
suspension
Morphology
round to polymorph cells growing singly and in clumps in suspension
STR DNA Profile
Amelogenin X
CSF1PO 12
D5S818 9,11
D7S820 10,11
D13S317 9,13
D16S539 9
TH01 6
TPOX 9,11
vWA 17
Karyotype
Human highly rearranged flat-moded hypertetraploid karyotype with 2.5% polyploidy; 84-96<4n>XXXX, +8, +8, +8, -9, t(5;6)(q21;q24)x2, der(7)t(4;7)(q31;q33)x2, der(7)dup(7)(q?31q?35)t(5;7)(p15;q35)x2, der(8)t(8;8)(p22;q24)inv(8)(q12q24)x2, der(9;10)(q10;q10
Markers
CD2 +, CD7 +, CD11a +, CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -, CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16 -, CD19 -, CD20 -, CD23 -, CD34 -, HLA-DR -
Sequence Variations
KRAS p.Gly12Ala (c.35G>C) TP53 p.Arg248Trp (c.742C>T)
Applications
a. Natural killer lymphoma related reserach
b. The study of enhanced cytotoxicity by NK cells
c. Cell-Based assay
d. In vivo efficacy study
Quality Control
Mycoplasma: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Selective biochemical test: negative
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Storage and Shipping
Ship in dry ice.
Store in liquid nitrogen.
BioSafety Level
BSL–1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
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