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KHYG-1

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Cat.No.
CSC-C0784
Description
Established 1997 from the peripheral blood of a 45-year-old woman with aggressive natural killer cell (NK) leukemia; cells were described to display strong cytotoxic activity and to carry a point mutation in exon 7 of the TP53 (p53) gene
Organism
Human
Karyotype
Human highly rearranged flat-moded hypertetraploid karyotype with 2.5% polyploidy; 84-96<4n>XXXX, +8, +8, +8, -9, t(5;6)(q21;q24)x2, der(7)t(4;7)(q31;q33)x2, der(7)dup(7)(q?31q?35)t(5;7)(p15;q35)x2, der(8)t(8;8)(p22;q24)inv(8)(q12q24)x2, der(9;10)(q10;q10
Source
natural killer cell leukemia
Culture Properties
suspension
Morphology
round to polymorph cells growing singly and in clumps in suspension
Recommended Medium
90% RPMI-1640 + 10% h.i. FBS+ 10 ng/ml IL-2
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 +, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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