JOSK-M

Cat.No.: CSC-C0208

Species: Homo sapiens (Human)

Source: Pleural Effusion

Morphology: single, round to oval cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0208

Description

Supposedly "established from the peripheral blood of a 37-year-old man with chronic myeloid leukemia (CML) in myelomonocytic blast crisis in 1984"; however, Creative Bioarray DNA fingerprinting and cytogenetic analysis established unequivocally that JOSK-I, JOSK-M and U-937 are identical; thus, JOSK-M is clearly a derivative of U-937

Species

Homo sapiens (Human)

Source

Pleural Effusion

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round to oval cells in suspension

Karyotype

Human hyperdiploid with 4% polyploidy - 55(51-57)<2n>XXY, +X, +3, +7, +8, +19, +20, +3mar, t(1;5;7)(p21-22;q35;p11), der(3)t(1;3)(q21;q26-27), der(6)t(6;?)(p25;?), der(10)t(10;?)(p13;?), del(11)(q22-23), der(12)t(12;?)(p13;?), der(15)t(15;?)(q24;?)

Disease

Acute Monocytic Leukemia

Quality Control

Mycoplasma: negative in DAPI, micobiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.