JOSK-I

Cat.No.: CSC-C0303

Species: Homo sapiens (Human)

Source: Pleural Effusion

Morphology: single, round to polygonal cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0303

Description

Supposedly "established from the peripheral blood of a 72-year-old woman with acute myelomonocytic leukemia (AML FAB M4) in 1983"; however Creative Bioarray DNA fingerprinting and cytogenetic analysis established unequivocally that JOSK-I, JOSK-M and U-937 are identical; thus, JOSK-I is clearly a derivative of U-937

Species

Homo sapiens (Human)

Source

Pleural Effusion

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round to polygonal cells growing in suspension

Karyotype

Human hyperdiploid karyotype with 3.5% polyploidy - 56(52-56)<2n>Xder(X), +2, +4, +6, +7, +8, +12, -15, +17, +19, +20, +mar, der(X)t(X;3)(q11;p13)t(1;3)(q11;p25), del(1)(p11p22), t(1;5)(p21.1;q23), t(2;18)(p17;q23), del(4)(q22), add(6)(p23), del(7)(q21)/d

Disease

Acute Monocytic Leukemia

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD68 +, HLA-DR +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.