JOSK-I

Specification
Recommended Products

Cat.No.

CSC-C0303

Description

Supposedly "established from the peripheral blood of a 72-year-old woman with acute myelomonocytic leukemia (AML FAB M4) in 1983"; however Creative Bioarray DNA fingerprinting and cytogenetic analysis established unequivocally that JOSK-I, JOSK-M and U-937 are identical; thus, JOSK-I is clearly a derivative of U-937

Species

Human

Source

histiocytic lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round to polygonal cells growing in suspension

Karyotype

Human hyperdiploid karyotype with 3.5% polyploidy - 56(52-56)<2n>Xder(X), +2, +4, +6, +7, +8, +12, -15, +17, +19, +20, +mar, der(X)t(X;3)(q11;p13)t(1;3)(q11;p25), del(1)(p11p22), t(1;5)(p21.1;q23), t(2;18)(p17;q23), del(4)(q22), add(6)(p23), del(7)(q21)/d

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD68 +, HLA-DR +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

Why do we use HEPES in cell culture?

HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Impressed consistency

I was impressed with the consistency of the product across multiple batches, which is the reason I have returned to the product many times.

13 Sep 2022

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